Nystatin-Induced Potassium Efflux from Saccharomyces cerevisiae Measured by Flame Photometry: a Potential Bioassay Approach

Nystatin-induced changes in Saccharomyces cerevisiae.

Nystatin (5 to 10 mug/ml) was fungicidal to Saccharomyces cerevisiae NCYC 361. There was an initial rapid uptake of nystatin by the yeast cells at 30 C, the uptake being pH dependent, with a maximum at low pH values (3 to 4). Photomicrographs indicated that nystatin-treated cells became granular. The effect of growth temperature on the subsequent sensitivity of the yeast cells to nystatin was i...

Interaction among Nystatin and Penicillin G against Saccharomyces cerevisiae

interaction among nystatin and penicillin g against saccharomyces cerevisiae

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Potassium efflux from myocardial cells induced by defibrillator shock.

A transient, dose-dependent cardiac depression was produced by defibrillator shocks in an isolated, working canine heart preparation perfused with oxygenated arterial blood from a support dog. Accompanying this depression was an efflux of potassium (K+), forced out of the myocardial cells by the passage of defibrillating current. The transient increase in extracellular K+ concentration was reco...

Continuous-recording flame photometry of sodium and potassium in serum.

Serum sodium and potassium analyses by continuous-flow flame photometry is a well-accepted procedure (1, 2). Recently Habig and Williamson (3) have used the widely available Instrumentation Laboratory Model 143 (IL-143) flame photometer for continuousflow analyses. Pennacchia et al. (4) have operated this flame photometer at rates as high as 120 samples per hour. In both systems the results app...